Part:BBa_K4728010

TpiA (Transmembrane Protein)

Originating from Comamonas sp. E6, TpiA is a part of the TpiA-TpiB transmembrane proteins. TpiA is constitutively expressed as a single operon in E6 cells, and it represents the large transmembrane protein. Together alongside TpiB, the TpiA-TpiB membrane components interact in conjunction with TphC for encoding a tripartite transporter required for TPA uptake, and involved in uptake of IPA, OPA, PCA, and citrate. The TpiA-TpiB system is dependent on the proton motive force, thus inhibitors of the protonophore result in complete inhibition of TPA uptake (Hosaka et al., 2013).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1432
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 781
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 358
  Illegal NgoMIV site found at 934
  Illegal AgeI site found at 1172
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

Hosaka, M., Kamimura, N., Toribami, S., Mori, K., Kasai, D., Fukuda, M., and Masai, E. (2013). Novel Tripartite Aromatic Acid Transporter Essential for Terephthalate Uptake in Comamonas SP. strain E6. Applied and Environmental Microbiology, 79(19), 6148-6155. https://doi.org/10.1128/AEM.01600-13